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Adiponectin and peroxisome proliferator-activated receptor gamma (pPARγ) variation after nerve lesion in AT. (A) Adiponectin measured by enzyme-linked immunosorbent assay <t>(ELISA)</t> in AT at baseline condition (BL) and 7 days after CCI (D7) in male and female mice. Unpaired t -test: BL M vs. D7 M t 4 = 3,879, p = 0.0179 (*); BL F vs. D7 F t 4 = 5,573, p = 0.0051 (*); BL M vs. BL F t 4 = 3,037, p = 0.0385 (°); D7 M vs. D7 F t 4 = −6,219, p = 0.0034 (°°); n = 3 group. (B) pPARγ measured by ELISA in AT at baseline condition (BL) and 7 days after CCI (D7) in male and female mice. Unpaired t -test: BL M vs. D7 M t 4 = 22,629, p < 0.0001 (***); BL F vs. D7 F t 4 = 15.071, p = 0.0001 (**); BL M vs. BL F t 4 = −5,452, p = 0.0055 (°); D7 M vs. D7 F t 4 = −96,779, p < 0.0001 (°°°); n = 3 group.
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Adiponectin and peroxisome proliferator-activated receptor gamma (pPARγ) variation after nerve lesion in AT. (A) Adiponectin measured by enzyme-linked immunosorbent assay (ELISA) in AT at baseline condition (BL) and 7 days after CCI (D7) in male and female mice. Unpaired t -test: BL M vs. D7 M t 4 = 3,879, p = 0.0179 (*); BL F vs. D7 F t 4 = 5,573, p = 0.0051 (*); BL M vs. BL F t 4 = 3,037, p = 0.0385 (°); D7 M vs. D7 F t 4 = −6,219, p = 0.0034 (°°); n = 3 group. (B) pPARγ measured by ELISA in AT at baseline condition (BL) and 7 days after CCI (D7) in male and female mice. Unpaired t -test: BL M vs. D7 M t 4 = 22,629, p < 0.0001 (***); BL F vs. D7 F t 4 = 15.071, p = 0.0001 (**); BL M vs. BL F t 4 = −5,452, p = 0.0055 (°); D7 M vs. D7 F t 4 = −96,779, p < 0.0001 (°°°); n = 3 group.

Journal: Frontiers in Pain Research

Article Title: Metabolic resilience governs sex-specific pain recovery during hormonal aging: a multi-omics study of neuropathy in mice

doi: 10.3389/fpain.2025.1655712

Figure Lengend Snippet: Adiponectin and peroxisome proliferator-activated receptor gamma (pPARγ) variation after nerve lesion in AT. (A) Adiponectin measured by enzyme-linked immunosorbent assay (ELISA) in AT at baseline condition (BL) and 7 days after CCI (D7) in male and female mice. Unpaired t -test: BL M vs. D7 M t 4 = 3,879, p = 0.0179 (*); BL F vs. D7 F t 4 = 5,573, p = 0.0051 (*); BL M vs. BL F t 4 = 3,037, p = 0.0385 (°); D7 M vs. D7 F t 4 = −6,219, p = 0.0034 (°°); n = 3 group. (B) pPARγ measured by ELISA in AT at baseline condition (BL) and 7 days after CCI (D7) in male and female mice. Unpaired t -test: BL M vs. D7 M t 4 = 22,629, p < 0.0001 (***); BL F vs. D7 F t 4 = 15.071, p = 0.0001 (**); BL M vs. BL F t 4 = −5,452, p = 0.0055 (°); D7 M vs. D7 F t 4 = −96,779, p < 0.0001 (°°°); n = 3 group.

Article Snippet: Leptin levels in serum and AT were quantified using the Mouse Leptin (LEP) ELISA Kit (BT LAB) according to the manufacturer's instructions, and absorbance values were obtained using the Varioskan Lux Instrument (ThermoFisher).

Techniques: Enzyme-linked Immunosorbent Assay